Analysis of high risk factors for chromosomal aberrations in peripheral blood lymphocytes of 200 radiation workers in Hainan Province / 上海预防医学

ObjectiveTo investigate the current status and high-risk factors of chromosomal aberrations in peripheral blood lymphocytes （PBL） of radiation workers in Hainan Province. MethodsA total of 200 radiological workers who underwent occupational health examination in Hainan Provincial Hospital of Traditional Chinese Medicine from January 2021 to December 2021 were selected to collect the occupational health examination data and the rate of PBL chromosomal aberrations. The influencing factors of PBL chromosomal aberrations were analyzed by logistic regression model. The predictive value of logistic regression prediction model on PBL chromosomal aberrations were determined by using the reciver operator characteristic （ROC） curve. ResultsA total of 20 000 cells （100 cells/person） were tested. The chromosomal aberration rate was 0.37% （74/20 000） and the PBL chromosomal aberration rate in the subjects was 6.00% （12/200）. Univariate analysis showed that PBL chromosomal aberrations in radiological workers were related to age， length of service， type of work and education （all P<0.05）， but not to gender （P>0.05）. The logistic regression prediction model was constructed based on the influencing factors， with χ2=9.413， df=9， P=0.852， suggesting a good model fit. The logistic regression prediction model predicted the area under the curve （AUC） for the occurrence of PBL chromosomal aberrations in radiation workers was 0.914 （95%CI： 0.866‒0.949）， with a cut-off value of 3.05， corresponding to a prediction sensitivity and specificity of 100.00% and 75.98%， respectively. ConclusionThe incidence of PBL chromosomal aberrations in radiological workers in Hainan Province was 6.00%， with age， working age and job type as high-risk factors and education level as a protective factor. The prediction model constructed by the above factors can provide a reliable basis for clinical prediction of PBL chromosomal aberrations in radiological workers.

Effect of exposure to low concentrations of benzene on miR-223 and miR-155 expression in peripheral blood lymphocytes / 预防医学

Objective @#To investigate the effect of exposure to low concentrations of benzene on miR-155 and miR-223 expression in peripheral blood lymphocytes among workers with benzene exposure. @*Methods @#A hundred male employees at a risk of exposure to benzene (the exposed group) were randomly sampled from two small metal products manufacturing enterprises and one medium-sized chemical raw material and chemical products manufacturing enterprise in Ningbo City, Zhejiang Province, and 60 age-matched male employees without benzene exposure were randomly selected as the unexposed group. Age, body mass index ( BMI ), smoking status, alcohol consumption, disease history, medication history and routine blood testing results of subjects were collected using a questionnaire survey. The 8-hour time weighted average concentration ( CTWA ) of benzene was measured in the workplace using thermal desorption gas chromatography, and the urine 8-hydroxy-2' deoxyguanosine ( 8-OHdG ) levels were determined using high-performance liquid-chromatography tandem mass spectrometry (HPLC-MS/MS). The miR-155 and miR-223 expression was quantified in peripheral blood lymphocytes using quantitative fluorescent reverse transcription-polymerase chain reaction assay, and the factors affecting miR-155 and miR-223 expression were identified using multivariable logistic regression analysis. @*Results @#The subjects in the exposed group had a mean age of ( 31.17±7.30 ) years, and were exposed to low concentrations of benzene ( CTWA, 0.05 to 0.30 mg/m3 ) , while the subjects in the unexposed group had a mean age of ( 32.52±6.15 ) years. There were no significant differences between the exposed and unexposed groups in terms of age, BMI, proportion of smokers or proportion of alcohol consumers ( P>0.05 ). There was no significant difference in the median relative miR-155 expression between the exposed and unexposed groups ( 0.953 vs. 1.293, P>0.05 ), and lower median relative miR-223 expression was quantified in the exposed group than in the unexposed group ( 0.540 vs. 1.433, P<0.05 ). Multivariable logistic regression analysis revealed that down-regulation of miR-223 expression correlated with exposure to benzene ( OR=2.719, 95%CI: 1.308-5.651 ). @*Conclusion @#Down-regulation of miR-223 expression may be associated with exposure to low concentrations of benzene.

Effect of HFRT and CFRT on peripheral blood lymphocytes in patients with breast cancer / 中国辐射卫生

Objective To investigate the effect of different fractionated radiotherapy of hypofractionated radiotherapy (HFRT) and conventional fractionated radiotherapy (CFRT) on peripheral blood lymphocytes in patients with breast cancer. Methods This retrospective analysis enrolled 40 patients with early breast cancer who underwent radiotherapy post breast conserving surgery in Xuzhou Central Hospital from November 2019 to August 2021. The patients were randomly divided into the observation group (HFRT, n = 20) and the control group (CFRT, n = 20). Changes in peripheral blood lymphocyte count (PLC) before and during radiotherapy were compared between the two groups. Results The baseline PLC was comparable between the observation group and the control group (1.53 ± 0.54 vs 1.64 ± 0.56, P > 0.05). In both groups, the PLC declined steadily during radiotherapy, and the incidence of lymphopenia in the observation group was lower than that in the control group (32.5% vs 50.0%, P > 0.05); the PLC nadir was higher in the observation group than in the control group (0.91 ± 0.28 vs 0.55 ± 0.22, P < 0.001). The ratio of the PLC nadir during treatment to baseline was significantly higher in the observation group than in the control group (0.64 ± 0.24 vs 0.38 ± 0.21, P < 0.05). Conclusion Patients with breast cancer receiving HFRT show a lower risk of radiation-induced lymphopenia versus those receiving CFRT.

Umbilical cord mesenchymal stem cells for the treatment of refractory chronic graft-versus-host disease / 中国组织工程研究

BACKGROUND: Chronic graft-versus-host disease is the most common complication after transplantation and glucocorticoid is a first-line drug. Glucocorticoid in combination with immunosuppressive therapy is not effective in half of the patients with hormone resistant chronic graft-versus-host disease. The low immunogenicity of umbilical cord mesenchymal stem cells provides the possibility for clinical treatment of graft-versus-host disease. OBJECTIVE: To investigate the clinical efficacy and safety of umbilical cord-derived mesenchymal stem cells to treat refractory chronic graft-versus-host disease. METHODS: Fifteen patients with refractory chronic graft-versus-host disease received mesenchymal stem cell infusion treatment based on immunosuppressive therapy. The therapeutic efficacy, infusion-related adverse reactions, and survival were analyzed. The ratio change of peripheral blood lymphocytes was determined by flow cytometry. This study was approved by Medical Ethics Committee, Third Affiliated Hospital of Sun Yat-sen University in China. RESULTS AND CONCLUSION: There were 12 male and 3 female patients with a median age of 29 years (ranging from 17 to 52 years). Four patients obtained complete response, seven patients obtained partial response, 11 had overall response, and four patients had no response. After treatment by umbilical cord mesenchymal stem cells, the ratio of CD19+ cells in the peripheral blood was slightly, but not significantly lower, but CD19+ CD27+ and CD3+ cell ratios were slightly, but not significantly higher than those before treatment. No patients had adverse reactions related to infusion of umbilical cord mesenchymal stem cells and no patients had primary disease recurrence and mesenchymal stem cell-related tumor. These findings suggest that umbilical cord-derived mesenchymal stem cell infusion is an effective and safe therapy for refractory chronic graft-versus-host disease.

Protective effect of luteolin on DNA damage of peripheral blood lymphocytes of isoproterenol-induced heart failure rats / 西安交通大学学报(医学版)

Objective: To explore the effects of luteolin on DNA damage of peripheral blood lymphocytes of isoproterenol-induced heart failure rats by using comet assay and micronucleus assay. Methods: Male SD rats aged 6-7 weeks were randomly divided into heart failure group (HF, n=10) and control group (Ctrl, n=10). The heart failure model was established through intraperitoneal injection of isoproterenol (50 mg/kg) for 10 consecutive days. Echocardiography was conducted to confirm HF model construction. Blood was taken from the abdominal aorta from rats in both groups using EDTA-anticoagulate tubes. Peripheral blood lymphocytes were separated bylymphocyte isolation kit within two hours after the blood was taken. Comet assay was carried out to test the short-run damage of DNA. Then those lymphocytes were treated with 5, 10, 20, and 50 μmol/L of luteolin and comet assay was carried out again after 30 min to test DNA damage. The lymphocytes were treated with 50 μmol/L of luteolin to culture new peripheral blood for micronucleus assay. Optic microscopy detected long-term DNA damage of the cells. Results: Compared with those in Ctrl group, the mean ejection fraction of left ventricle (EF%) of HF group was decreased; left ventricular fraction shortening, left ventricular end-systolic diameter, and left ventricular end-diastolic diameter significantly increased. These indicated that the heart failure model was established successfully. Compared with Ctrl group, the ratio of Tail DNA in HF group increased (P<0.05). Compared with that in HF group, Tail DNA decreased in 50 μmol/L luteolin-treated HF group (P<0.05). The micronuleus assay results were in accordance with comet assay results. Basically, the number of micronucleus in 1000 cells increased in HF group compared with Ctrl group, but decreased when treated with 50 μmol/L of luteolin. Conclusion: Luteolin can significantly reduce DNA damage on peripheral blood lymphocytes of HF rats.

Peripheral blood absolute lymphocyte count, absolute monocyte count and their ratio in prognosis prediction of newly diagnosed multiple myeloma / 肿瘤

Objective: To investigate the value of peripheral blood absolute lymphocyte count (ALC), absolute monocyte count (AMC) and ALC/AMC ratio (LMR) in predicting the prognosis of patients with newly diagnosed multiple myeloma (MM). Methods: Retrospective analysis was performed in 190 patients with newly diagnosed MM from Tianjin Medical University Cancer Institute and Hospital between January 2005 and December 201 5. The relationships of peripheral blood ALC, AMC and LMR with peripheral blood hemoglobin (Hb), β2-microglobulin (β2-MG) and lactate dehydrogenase (LDH) and the proportion of bone marrow plasma cells were analyzed. The cutoff values of ALC, AMC and LMR were determined by the receiver operating characteristic (ROC) curve in patients with newly diagnosed MM. Survival analysis was performed using Kaplan-Meier method and the log-rank test for univariate analysis of prognosis, and a COX proportional hazard model was used for multivariate analysis of prognosis. Results: The cutoff values of ALC, AMC and LMR determined by ROC curve were 1.24×109/L, 0.60×109/L and 3.90, respectively. According to these cutoff values, the patients were divided into high value groups and low value groups. The results of multivariate analysis showed that ALC 247 U/L (HR: 1.972, 95% CI: 1.087-3.576; P = 0.025) were independent poor prognostic factors in untreated MM patients. According to the number of poor prognostic factors (each poor prognostic factor was scored as one), the patients were divided into score 0, 1-2 and 3 groups, the overall survival and progression-free survival of the three groups were significantly different (all P < 0.05). Conclusion: Lower ALC and LMR values may indicate poor prognosis. ALC < 1.24×109/L and LMC≤3.90 maybe the independent poor prognostic factors in patients with newly diagnosed MM.

Expression of Epstein-Barr Virus DNA in Peripheral Blood Lymphocytes and Plasma in Patients with EBV-Associated Diseases / 现代检验医学杂志

Objective To explore the expression of Epstein-Barr virus DNA(EBV DNA)in the peripheral blood lymphocytes and plasma of patients with EBV-associated diseases.Methods The whole blood samples were collected from 112 patients with suspected EB virus infection diseases,including 14 cases of nasopharyngeal carcinoma(NPC),16 cases of infectious mononucleosis(IM),22 cases of lymphoma,23 cases of autoimmune disease,26 cases of upper respiratory tract infection, and 11 cases of abnormal liver function.The levels of EBV DNA in lymphocytes and plasma of the same sample were detec-ted by fluorescence quantitative PCR(FQ-PCR).Results The EBV DNA positive rates in lymphocytes and plasma of all 112 patients were 83.0%(93/112)and 27.7%(31/112)respectively,with statistically significant difference(χ2=60.02,P<0.01).The positive rate and the load of EB virus DNA in lymphocytes and plasma of 14 patients with nasopharyngeal car-cinoma(NPC)had no statistical difference(χ2=2.25,t=-1.04,all P>0.05).However,patients with lymphoma,infec-tious mononucleosis,upper respiratory tract infection,autoimmune disease or abnormal liver function,the positive rates and the concentration of EBV DNA in the plasma were dramatically lower than those in the peripheral blood lymphocytes,and the difference was statistically significant(χ2=4.17~15.06,all P<0.05;t=3.94~10.45,all P<0.01).Conclusion The detection of EB DNA in peripheral blood lymphocytes of non NPC patients by FQ-PCR might be better than that in plasma. There was no statistical difference between the detection of EBV DNA in lymphocytes and plasma of patients with nasopha-ryngeal carcinoma.Appropriate specimen type could be selected according to clinical consideration.

Study on chemosensitivity test in vitro between peripheral blood lymphocytes and tumor cells in elderly lung cancer / 中国基层医药

Objective To explore the correlation of chemosensitivity in vitro of anti-cancer drugs between peripheral blood lymphocytes and tumor cells in non-small cell lung cancer of elderly patients.Methods The MTT method was used to test the sensitivity of the peripheral blood lymphocyte and the tumor cells of 52 patients with nonsmall cell lung cancer to 13 kinds of anti-cancer drugs.Results The sensitivity test in eleven drugs include CDDP,CBP,LOHP,PTX,CTX,ICTX,THP,VP-16,GEM,VCR and NVB in the peripheral blood lymphocyte were associated with that in the tumor cells.But no dependablity in two drugs,ADM and HCPT.Conclusion The peripheral blood lymphocytes could be replace tumor cells to test the chemosensitivity of anti-cancer drugs in patients with non-small cell lung cancer.

Clinical significance of lymphocyte subsets changes in the active stage of ulcerative colitis: a retrospective study / 中华临床营养杂志

Objective To observe the change of peripheral blood lymphocytes subsets in patients with active ulcerative colitis (UC) and explore its clinical significance. Methods The clinical data and lymphocyte subsets of 40 active UC patients who admitted to the Department of Gastroenterology, Peking University First Hospital,from June 2007 to January 2010 were retrospectively analyzed. Patients who had previously used immunosuppressants or tumor necrosis factor monoclonal antibody were excluded. Seventy-nine subjects with health examination served as controls. Peripheral blood lymphocyte subsets detected included total T cells, CD4 + T cells, CD8 + T cells, B cells, and NK cells. Only the first detection results of UC patients were used for analysis. Results The proportion of total T cells for UC patients (73.60% ± 8.35% ) was significantly higher than the controls (69. 76% ±7.37%) (P =0.012). CD8+T cell ratio (35.53% ± 10.99%) was significantly higher than the controls ( 30. 56% ± 6. 75 % ) ( P = 0. 011 ). When the UC patients were stratified according to inflammatory involvement,the total T cells, CD8 +T cell, and NK cell ratio were significantly different among pancolitis, non-pancolitis, and the controls ( all P ＜ 0. 05 ). When the UC patients were stratified according to the disease course, the total T cells and CD8 + T cell ratio were significantly different among chronic recurrent/persistent, onset, and the controls ( both P ＜ 0.05 ). When the UC patients were stratified according to the extraintestinal manifestations, the total T cells and CD8 + T cell ratio were significantly different among those with extraintestinal manifestations, those without extraintestinal manifestations, and the controls ( both P ＜ 0.05 ). Conclusions The proportion of total T cells and CD8 + T cells increase in UC patients. Such immune abnormalities are even more distinctive in those UC patients who suffer from pancolitis or chronic recurrent/persistent type or those with extraintestinal manifestations.

The Unscheduled Expression of Cyclin B1/CDK1 Contributes to the Transition of Cell Cycle of Lymphocytes in vivo from G_0 to G_1 Phase / 华中科技大学学报(医学版)

Objective To study the mechanism of unscheduled Cyclin B1 expression at G_1 phase which is usually at G_2/M phase.Methods Human peripheral blood lymphocytes(PBL) from healthy volunteers were firstly activated by PHA and then went into cell cycle.The cells were collected at 0,36,48 and 60 h after activation and divided into two parts:one for Cyclins/DNA muhiparameter assay,and another for Post-sorting Western blot.Results After activation by PHA,Cyclin B1 and CDK1 of lymphocytes were expressed at G_1 phase.Conclusion Unscheduled Cyclin B1/CDKl probably contributes to lymphocytes in vivo into cell cycle.

Apoptosis and Expression of Caspase in Peripheral-blood Lymphocyte(PBLC) of Systemic Lupus Erythematosus(SLE) Patients / 医学研究杂志

Objective To study the apoptosis of PBLC in SLE patients and the roles of caspase in the pathogeneses of SLE.Methods PBLC apoptosis was evaluated by vitro cell culture in 25 patients with SLE(11 active stage,14 catabasis),and 18 controls.The expression of caspase of PBLC was also detected by Western blot.Results In SLE,compared with controls,the apoptosis of PBLC were reduced before cell culture while those were increased after culture 24 and 48hrs.Expressions of caspase-2,3 in active stage patients were lower than those of catabasis group and controls.Conclusions Abnormal PBLC apoptosis results in decreasing Caspase2,3 in SLE patients.

Detection of HBV cccDNA in Peripheral Blood Lymphocyte / 医学研究杂志

Objective Assaying HBV cccDNA in periphral blood lymphocyte and discuss its clinical significance.Methods Using lymphocyte isolating solution to isolate lymphocytes in the peripheral blood, and quantitative florescent realtime PCR was used to detect HBV cccDNA.Results 29 cases of 36 CAH patients was detected positive(80.56%),32 cases of 61 CPH patients was detected positive(52.46%) and 1 case of 59 ASC was detected positive(0.02%) respectively.All 47 healthy people specimen was found negative by the method.CAH group,CPH group and ASC & negative control group show significance statistically(P

The immune suppressive effect from the stress of maxillofacial operations

Suppression of cellular immunity is the host responses to surgical stress. When the body is exposed to surgical stress, decreased immunocyte function is one of the surgical stress-induced biologic responses. In all patients exposed to the surgical stress, peripheral blood lymphocyte numbers and function were suppressed until at least 2 weeks postoperatively. This immunosuppression was mainly due to a decrease of helper-inducer T cells, cytotoxic T cells, natural killer cells, and an increase of suppressor T cells. The blood levels of interleukin-6(IL-6) cytokine increase in response to surgical stress and cause an increase of so-called acute phase reactants, including C-reactive protein(CRP). In the previously damaged patients group, expected to early stress expose, immunosuppression was more developed than other normal groups. Cellular immunosuppression by surgical stress was mainly due to an increase of lymphocyte subsets that depress cellular immunity coupled with a decrease of the subsets that promote it. Overproduction of CRP in response to surgical stress may play an important role in the development of immunosuppression.

Characterization of the nover late T cell activation proteins, 519 and 520

The late T cell activation gene, 519, is expressed in antigen specific, growth factor dependent T cell lines and clones but not in T or B cell tumors, other hematopoietic cells, tonsil, muscle, lung, or liver. Resting peripheral blood lymphocytes (PBLs) express little or no 519mRNA, but levels increase dramatically 5~7 days after activation with alloantigen or mitogen. Four alternatively spliced transcripts of the gene exist. 520 cDNA, the predominate gene transcript, encodes a protein of 144 amino acids and has a potentially cleavable hydrophobic leader of 15 amino acids at the amino terminus. The 519 transcript encodes a 129 amino acid product. An polyclonal antiserum was produced by immunizing a rabbit with a synthetic peptide corresponding to the second hydrophobic region common to both 519 and 520. 14.3 and 15.5kD protein bands were immunoprecipitated (IP) from radiolabled in-vitro translated products, 519 and 520 respectively. A protein of 14.3kD was detected by IP of in-vivo 35S cysteine and methionine radiolabled cytotoxic T lymphocytes (CTL) cells. Western blotting of lysates from a CTI line and a B cell tumor line revealed a distinct band at 14.3kD only in CTL lanes. Both CTL and peripheral blood lymphocytes expressed the greatest amount of 519/520 protein in cells 5~7 days after stimulation. Western blotting of differential centrifugation samples localized 519/520 protein to the granule layer. Comparison of 519/520 proteins with other known protin sequences identified homology with surfactant associated and sphyngolipid activating proteins. A homologous gene(NKC5) is present in natural killer cells (NK). The increase in the amount of the 519/520 protein product in CTL and PBL parallel increases in mRNA expression. 519/520 and related gene products are present in CTL, PBL and NK cells, have structural similarity with lipid associating proteins.

Genotoxicity of 1-Nitropyrene to Human Peripheral Blood Lymphocytes in vitro / 环境与健康杂志

0.05), but were highly significant at doses of 100 ?mol/L or above (P

Effect of methionine-enkephalin on proliferation and mechanism involved by opiate receptor of peripheral blood lymphocyte from patients with systemic lupus erythematosus / 中国药理学通报

AIM\ To observe the effect of methionine enkephalin(Met Enk) on proliferation and mechanism involved by opiate receptor of peripheral blood lymphocyte(PBL) from patients with systemic lupus erythematosus(SLE). METHODS\ Lymphocyte proliferation assay. RESULTS\ Naloxone (Nal,1?10 -6 mol?L -1 )could block the increasing effect of Met Enk(1?10 -8 ～1?10 -6 mol?L -1 )on the PBL proliferation of normal humans,but there was no direct effect on that. However,Nal could not only block the promotive action of Met Enk on PBL proliferation of SLE patients,but there was a direct decreasing effect on it. CONCLUSION\ Endogenous Met Enk may participate in the abnormal regulation with SLE PBL via opiate receptor.

Study of proliferative reaction of erythrocyte promoting peripheral blood lymphocyte on chronic renal failure patients / 中国免疫学杂志

Objective:To study the activity of immunological regulative function of red blood cells of the patients with chronic renal failure.Methods:The proliferative reaction of the erythrocyte promoting lymphocyte was detected in CRF patients and healthy persons with MTT colorimetry,in which the normal person's lymphocyte as the target cell and the erythrocyte as the stimulating cell.Results:It was found that the healthy persons of control group had obvious proliferative reaction of the erythrocyte promoting lymphocyte(promoting rate:57.3%?10.2%),while the 36 CRF patients had lower proliferative activity(promoting rate:32.7%?7.8%,P

Study on chemiluminescence of human peripheral blood lymphocytes / 中国免疫学杂志

Lymphocyte chemiluminescence (Ly-CL) is one of the early events involved in the activation of lymphocytes. This CL is thought to be result ed from the reactive oxygen species (O_2, H_2 O_2, OH, 'O_2) produced by the lymphocytes, the consequent oxidation of luminol and emission of light. In this paper we investigated the experimental condition for ConA-induced human peripheral blood lymphocyte (PBL) CL by using orthogonal design. The effects of new born calf serum (NCS), bovine serum albumin (BSA), temperature and time of cell storage on CL were also studied. The results show that the optimal condition of Ly-CL assay was 1.0 ml PBL suspension (1.4 ? 10~6 cell/ml ), 0.2 ml Luminol solution ( 7 ? 10~(-4)M) and 0.2 ml ConA ( 700 ?g /ml ). The isolated PBL were suspended in phenol red-free Hanks solution containing 0.1% BSA and can be kept for 2 hr at 4℃ before being used without adversely affecting cell viability and CL.